Protein Methods

Protein Methods by Daniel M. Bollag, Michael D. Rozycki, and Stuart J. Edelstein offers an exemplary overview of essential protein techniques. The original reviews for the first edition praise its clarity and practicality:

"...it is very well written...would certainly be of use to undergraduate and postgraduate students entering the complex world of protein preparation and to more experienced scientists entering this field for the first time." - Biotechnology and Applied Biochemistry

"The book presents these topics with loads of practical detail, so that the reader has little need to consult other reference sources to carry out the techniques described. All in all, a useful book..." - Theoretical & Applied Genetics

"...clearly written with protocols that are easy to follow and the text is well spread-out and easy to read...all methods are fully referenced...a useful book for beginners at a reasonable price." - FEBS Letters

This revised and expanded Second Edition of Protein Methods remains the primary source for a comprehensive summary of tested and proven protein techniques. The book is now divided into two sections, beginning with essential updates from the first edition that reflect significant changes in methodology.

The chapter on protein isolation introduces a new section focusing on the isolation of proteins from inclusion bodies. In addition, the second section includes four new chapters dedicated to protein purification and crystallisation. The chapters cover topics such as:

• Preparation for Protein Isolation
• Protein Extraction and Solubilisation
• Protein Concentration Determination
• Concentrating Protein Solutions
• Gel Electrophoresis Under Denaturing Conditions
• Gel Electrophoresis Under...

Each chapter features:

• Reagent items and equipment required
• "View-at-a-glance" tabs
• Illustrations for all gel applications and other methods

Also included are complete supplier lists, appendices of basic chemical parameters and molecular data, key references, descriptive tables, and safety notes.